DNA shuffling

Directed evolution of thymidine kinase for AZT phosphorylation using DNA family shuffling

Fred C. Christians, Léonardo Scapozza, Andreas Crameri, Gerd Folkers, Willem P.C. Stemmer

Citations: 127 • 1999

Advances in directed protein evolution by recursive genetic recombination: applications to therapeutic proteins

Aaron L. Kurtzman, Sridhar Govindarajan, Katherine Vahle, Jennifer T. Jones, Volker Heinrichs, Phillip A. Patten

Citations: 111 • 2001

Large-scale robot-assisted genome shuffling yields industrial Saccharomyces cerevisiae yeasts with increased ethanol tolerance

Tim Snoek, Martina Picca Nicolino, Stefanie Van den Bremt, Stijn F. L. Mertens, Veerle Saels, Alex Verplaetse, Jan Steensels, Kevin J. Verstrepen

Citations: 105 • 2015

Directed evolution of a maltogenic α-amylase from Bacillus sp. TS-25

A. Daniel Jones, Michael Lamsa, Torben P. Frandsen, T. Spendler, Paul Harris, Alan Sloma, Fan Xu, J NIELSEN, J.A. CHERRY

Citations: 64 • 2008

Automated Purification of His ₆ -Tagged Proteins Allows Exhaustive Screening of Libraries Generated by Random Mutagenesis

Thomas Lanio, Albert Jeltsch, Alfred Pingoud

Citations: 24 • 2000

Robotic workflows for automated long-term adaptive laboratory evolution: improving ethanol utilization by Corynebacterium glutamicum

Lars Halle, Niels Hollmann, Niklas Tenhaef, Lea Mbengi, Christiane Glitz, Wolfgang Wiechert, Tino Polen, Meike Baumgart, Michael Bott, Stephan Noack

Citations: 15 • 2023

Automating Gene Library Synthesis by Structure-Based Combinatorial Protein Engineering

Melissa Dokarry, Caroline Laurendon, Paul E. O’Maille

Citations: 8 • 2012

Sequence-Independent Construction of Ordered Combinatorial Libraries with Predefined Crossover Points

Laetitia Jézéquel, Jacqueline Loeper, Denis Pompon

Citations: 6 • 2008