Recombinant DNA

Printing Proteins as Microarrays for High-Throughput Function Determination

Gavin MacBeath, Stuart L. Schreiber

Citations: 2764 • 2000

Antibody arrays for high-throughput screening of antibody–antigen interactions

Ruud M. T. de Wildt, Chris R. Mundy, B.D. Gorick, Ian M. Tomlinson

Citations: 627 • 2000

A New Vector for High-Throughput, Ligation-Independent Cloning Encoding a Tobacco Etch Virus Protease Cleavage Site

Lucy Stols, Minyi Gu, Lynda Dieckman, Rosemarie Raffen, F. Collart, Mark I. Donnelly

Citations: 541 • 2002

Profiling the humoral immune response to infection by using proteome microarrays: High-throughput vaccine and diagnostic antigen discovery

D. Huw Davies, Xiaowu Liang, Jenny E. Hernandez, Arlo Randall, Siddiqua Hirst, Yunxiang Mu, Kimberly M. Romero, Toai Nguyen, Mina Kalantari‐Dehaghi, Shane Crotty, Pierre Baldi, Luis P. Villarreal, Philip L. Felgner

Citations: 421 • 2005

A method for global protein expression and antibody screening on high- density filters of an arrayed cDNA library

Konrad Büssow

Citations: 282 • 1998

One-Step Purification of Recombinant Proteins Using a Nanomolar-Affinity Streptavidin-Binding Peptide, the SBP-Tag

Anthony D. Keefe, David Sloan Wilson, Burckhard Seelig, Jack W. Szostak

Citations: 264 • 2001

Protein expression systems for structural genomics and proteomics

Shigeyuki Yokoyama

Citations: 256 • 2003

Strategies to maximize heterologous protein expression in Escherichia coli with minimal cost

Wolfgang Peti, Rebecca Page

Citations: 227 • 2006

New baculovirus expression tools for recombinant protein complex production

Simon Trowitzsch, Christoph Bieniossek, Yan Nie, Frédéric Garzoni, Imre Berger

Citations: 220 • 2010

Robo-Lector – a novel platform for automated high-throughput cultivations in microtiter plates with high information content

Robert Huber, Daniel Ritter, Till Hering, Anne-Kathrin Hillmer, Frank Kensy, Carsten H. G. Müller, Le Wang, Jochen Büchs

Citations: 147 • 2009

MacroBac: New Technologies for Robust and Efficient Large-Scale Production of Recombinant Multiprotein Complexes

Scott Gradia, Justin P. Ishida, Miaw-Sheue Tsai, Chris Jeans, John A. Tainer, Jill O. Fuss

Citations: 143 • 2017

A Human cDNA Library for High-Throughput Protein Expression Screening

Konrad Büssow, Eckhard Nordhoff, Christine Lübbert, Hans Lehrach, Gerald Walter

Citations: 139 • 2000

Preparation and screening of an arrayed human genomic library generated with the P1 cloning system.

Nancy S. Shepherd, B D Pfrogner, John N. Coulby, Susan L. Ackerman, Ganesh Vaidyanathan, Rolf Sauer, T C Balkenhol, Nat Sternberg

Citations: 130 • 1994

Directed evolution of thymidine kinase for AZT phosphorylation using DNA family shuffling

Fred C. Christians, Léonardo Scapozza, Andreas Crameri, Gerd Folkers, Willem P.C. Stemmer

Citations: 127 • 1999

A High Through-put Platform for Recombinant Antibodies to Folded Proteins

Michael Hornsby, Marcin Paduch, Shane Miersch, Annika Sääf, Tet Matsuguchi, Brian H. Lee, Karolina Wypisniak, Allison K. Doak, Daniel A. King, Svitlana Usatyuk, Kimberly J. Perry, Vince Lu, William D. Thomas, Judy Luke, Jay S. Goodman, Robert J. Hoey, Darson Lai, Carly Griffin, Zhijian Li, Franco J. Vizeacoumar, Debbie Dong, Elliot Campbell, Stephen K. Anderson, Nan Zhong, S. Gräslund, Shohei Koide, Jason Moffat, Sachdev S. Sidhu, Anthony A. Kossiakoff, James A. Wells

Citations: 120 • 2015

An automated method for high-throughput protein purification applied to a comparison of His-tag and GST-tag affinity chromatography

Christoph Scheich, Volker Sievert, Konrad Büssow

Citations: 119 • 2003

Synthetic enzyme mixtures for biomass deconstruction: Production and optimization of a core set

Goutami Banerjee, Suzana Car, John S. Scott‐Craig, Melissa S. Borrusch, Nighat Aslam, Jonathan D. Walton

Citations: 118 • 2010

Robots, pipelines, polyproteins: Enabling multiprotein expression in prokaryotic and eukaryotic cells

Lakshmi Sumitra Vijayachandran, Cristina Viola, Frédéric Garzoni, Simon Trowitzsch, Christoph Bieniossek, Maxime Chaillet, Christiane Schaffitzel, Didier Busso, Christophe Romier, Arnaud Poterszman, Timothy J. Richmond, Imre Berger

Citations: 105 • 2011

Immobilized metal-affinity chromatography protein-recovery screening is predictive of crystallographic structure success

Ryan Choi, Angela Kelley, D.J. Leibly, Stephen N. Hewitt, Alberto J. Napuli, Wesley Van Voorhis

Citations: 103 • 2011

Investigation into the use of C‐ and N‐terminal GFP fusion proteins for subcellular localization studies using reverse transfection microarrays

Ella Palmer, Tom C. Freeman

Citations: 103 • 2004