Keith T. Demarest
Papers
1
Total Citations
8
H-Index
1
About
Keith T. Demarest has made significant contributions to the field of G protein-coupled receptor (GPCR) pharmacology, with a particular focus on the human adenosine A2a receptor, a key therapeutic target for neurological and inflammatory conditions. His most cited work, from 2004, details the development of a sensitive, high-throughput screening (HTS)-compatible reporter gene assay for functional analysis of the A2a receptor in CHO-K1 cells. This assay, which leverages the receptor's coupling to stimulatory G proteins and subsequent adenylate cyclase activation, provided a robust and scalable platform for drug discovery, enabling efficient evaluation of agonists and antagonists. With 8 citations, this paper has served as a foundational reference for researchers seeking to optimize GPCR assays for HTS applications. Demarest's work exemplifies the integration of molecular pharmacology and assay development, advancing the study of adenosine signaling and its therapeutic potential. His contributions remain relevant for scientists exploring GPCR-based drug targets and assay technologies.
Research Focus
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Top Papers
- 1