Brandon T. Ruotolo
Papers
2
Total Citations
23
H-Index
2
About
Brandon T. Ruotolo is a distinguished analytical chemist and structural biologist whose research sits at the cutting edge of native mass spectrometry and ion mobility techniques applied to complex biomolecular systems. His work has significantly advanced the field of native ion mobility-mass spectrometry (nIM-MS), a powerful technology for probing the three-dimensional architecture of proteins and multiprotein complexes in near-physiological conditions. Ruotolo's development of collision-induced unfolding (CIU) methodologies has provided researchers with unprecedented insight into biomolecular stability and conformational dynamics, enabling rapid structural evaluation that complements traditional structural biology approaches. His 2023 contribution introducing automated, high-throughput nIM-MS and CIU workflows represents a landmark achievement, streamlining what were once laborious experimental processes and making these powerful techniques more broadly accessible. Earlier foundational work, including his robotically assisted titration platform coupled to ion mobility-mass spectrometry, demonstrated innovative strategies for mapping multiprotein complex topologies, addressing a critical gap between high-throughput functional genomics and structural characterization. Together, these contributions — garnering recognition across the structural biology and analytical chemistry communities — underscore Ruotolo's enduring commitment to developing transformative tools that illuminate the structural complexity underpinning biological function.
Research Focus
Key Achievements
Top Papers
- 1
- 2