Target protein

One-Step Purification of Recombinant Proteins Using a Nanomolar-Affinity Streptavidin-Binding Peptide, the SBP-Tag

Anthony D. Keefe, David Sloan Wilson, Burckhard Seelig, Jack W. Szostak

引用数: 264 • 2001

An improved method for the in vitro evolution of aptamers and applications in protein detection and purification

Michael Murphy

引用数: 202 • 2003

An automated method for high-throughput protein purification applied to a comparison of His-tag and GST-tag affinity chromatography

Christoph Scheich, Volker Sievert, Konrad Büssow

引用数: 119 • 2003

A General Chemical Ligation Approach Towards Isopeptide‐Linked Ubiquitin and Ubiquitin‐Like Assay Reagents

Paul P. Geurink, Farid El Oualid, Anika M. Jonker, Dharjath S. Hameed, Huib Ovaa

引用数: 102 • 2011

High-throughput Screening of Surface Displayed Gene Products

Gerald Walter, Zoltán Konthur, Hans Lehrach

引用数: 58 • 2012

Optimized protocols for the isolation of specific protein-binding peptides or peptoids from combinatorial libraries displayed on beads

Thomas Kodadek, Kiran Bachhawat‐Sikder

引用数: 52 • 2005

A combinatorial systematic evolution of ligands by exponential enrichment method for selection of aptamer against protein targets

Bhairab Mondal, Shylaja Ramlal, Padma Sudha Rani Lavu, Harishchandra Sreepathy Murali, Harsh Vardhan Batra

引用数: 30 • 2015

High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in <em>E. coli</em>

Natalie J. Saez, Hervé Nozach, Marilyne Blémont, Renaud Vincentelli

引用数: 29 • 2014

Automation and Optimization of Protein Expression and Purification on a Novel Robotic Platform

Matthew Wollerton, Richard Wales, Jonathan A. Bullock, Ian R. Hudson, Mark Beggs

引用数: 18 • 2006

A fully automated three-step protein purification procedure for up to five samples using the NGC chromatography system

Wolfgang Becker, Anne Scherer, Christine Faust, David Kornblüh Bauer, Simone Scholtes, Ercole Rao, Joachim Hofmann, Rolf Schauder, Thomas Langer

引用数: 18 • 2018

Automated Microscale His‐tagged Protein Purification Using Ni‐NTA Magnetic Agarose Beads

Jianxin Meng, Johanna‐Gabriela Walter, Öznur Kökpınar, Frank Stahl, Thomas Scheper

引用数: 13 • 2008

High‐throughput protein purification under denaturating conditions by the use of cation exchange chromatography

Tove Alm, Johanna Stéen, Jenny Ottosson, Sophia Hober

引用数: 10 • 2007

A High-Throughput System for Transient and Stable Protein Production in Mammalian Cells

Sarah Rue, Paul W. Anderson, Michelle R. Gaylord, Scott Glaser, Scott A. Lesley

引用数: 10 • 2019

Bifunctional robots inducing targeted protein degradation

M. Elizabeth Sobhia, Harish Kumar, Sonia Kumari

引用数: 10 • 2023

Binding‐Site Purification of Actives (B‐SPA) Enables Efficient Large‐Scale Progression of Fragment Hits by Combining Multi‐Step Array Synthesis With HT Crystallography

Harold Grosjean, Anthony Aimon, Storm Hassell‐Hart, Warren Thompson, L. Koekemoer, James M. Bennett, A.R. Bradley, C.A. Anderson, Conor Wild, W.J. Bradshaw, Edward A. Fitzgerald, T. Krojer, O. Fedorov, Philip C. Biggin, John Spencer, F. von Delft

引用数: 9 • 2025

Peptide-based protein capture agents with high affinity, selectivity, and stability as antibody replacements in biodetection assays

Matthew B. Coppock, Blake Farrow, Candice R. Warner, Amethist S. Finch, Bert Lai, Deborah A. Sarkes, James R. Heath, Dimitra N. Stratis‐Cullum

引用数: 9 • 2014

Protein Arrays From cDNA Expression Libraries

Hendrik Weiner, Thomas Faupel, Konrad Büssow

引用数: 9 • 2004

A strategy for high-throughput screening of ligands suitable for molecular imprinting of proteins

Stefan Eppler, Tim Schröder, J. Friedle, Simone Michl, Werner Dangel, Boris Mizaikoff

引用数: 4 • 2012

Feedback-System-Control Integrated Microfluidic System for Fast Screening of Protein Crystallization Conditions

Sankhya Bhattacharya, Pijus Kundu, J.S. Liu, Wen‐Ching Wang, Fan‐Gang Tseng

引用数: 3 • 2020

Analysis of Protein Functions Through a Bacterial Cell-Free Protein Expression System

T. Kigawa

引用数: 2 • 2009