Protein engineering

Protein and antibody arrays and their medical applications

Dolores J. Cahill

Citations: 286 • 2001

Protein expression systems for structural genomics and proteomics

Shigeyuki Yokoyama

Citations: 256 • 2003

Machine Learning on a Robotic Platform for the Design of Polymer–Protein Hybrids

Matthew Tamasi, Roshan Patel, Carlos H. Borca, Shashank Kosuri, Heloise Mugnier, Rahul Upadhya, N. Sanjeeva Murthy, Michael E. Webb, Adam J. Gormley

Citations: 173 • 2022

Self-driving laboratories to autonomously navigate the protein fitness landscape

Jacob Rapp, Bennett J. Bremer, Philip A. Romero

Citations: 169 • 2024

Robotic Cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Thomas Acton, Kristin C. Gunsalus, Rong Xiao, Li Chung, James M. Aramini, Michael Baran, Yiwen Chiang, Teresa Climent, B. Cooper, Natalia G. Denissova, Shawn M. Douglas, J.K. Everett, Chi Kent Ho, Daphne Macapagal, P.K. Rajan, Ritu Shastry, Liang-Yu Shih, G.V.T. Swapna, Michael Wilson, Margaret Wu, Mark Gerstein, Masayori Inouye, J.F. Hunt, G.T. Montelione

Citations: 130 • 2005

Backbone flexibility in computational protein design

Daniel J. Mandell, Tanja Kortemme

Citations: 111 • 2009

Advances in directed protein evolution by recursive genetic recombination: applications to therapeutic proteins

Aaron L. Kurtzman, Sridhar Govindarajan, Katherine Vahle, Jennifer T. Jones, Volker Heinrichs, Phillip A. Patten

Citations: 111 • 2001

Fully automatized high‐throughput enzyme library screening using a robotic platform

Mark Dörr, Michael P. C. Fibinger, Daniel Last, Sandy Schmidt, Javier Santos‐Aberturas, Dominique Böttcher, Anke Hummel, Clare Vickers, Moritz Voß, Uwe T. Bornscheuer

Citations: 102 • 2016

High Throughput Production of Recombinant Human Proteins for Crystallography

O. Gileadi, N. Burgess-Brown, S. Colebrook, G. Berridge, P. Savitsky, C. Smee, P. Loppnau, C. Johansson, E. Salah, N. Pantic

Citations: 100 • 2008

Protein engineering via Bayesian optimization-guided evolutionary algorithm and robotic experiments

Ruyun Hu, Lihao Fu, Yongcan Chen, Junyu Chen, Yu Qiao, Tong Si

Citations: 60 • 2022

Focused Directed Evolution of Pentaerythritol Tetranitrate Reductase by Using Automated Anaerobic Kinetic Screening of Site‐Saturated Libraries

Martyn Hulley, Helen S. Toogood, Anna Fryszkowska, David Mansell, Gill Stephens, John M. Gardiner, Nigel S. Scrutton

Citations: 58 • 2010

Gel microdroplet–based high-throughput screening for directed evolution of xylanase-producing Pichia pastoris

Chunxuan Ma, Zheng Lin Tan, Ying Lin, Shuangyan Han, Xin‐Hui Xing, Chong Zhang

Citations: 53 • 2019

Human antibody libraries: A race to engineer and explore a larger diversity

Philippe Mondon

Citations: 53 • 2007

Heterologous expression of Melanocarpus albomyces cellobiohydrolase Cel7B, and random mutagenesis to improve its thermostability

Sanni Voutilainen, Harry Boer, Markus B. Linder, Terhi Puranen, Juha Rouvinen, Jari Vehmaanperä, Anu Koivula

Citations: 45 • 2007

Imaging-Based Screening Platform Assists Protein Engineering

Arne Fabritius, David Ng, Andreas M. Kist, Mutlu Erdoğan, Rubén Portugues, Oliver Griesbeck

Citations: 33 • 2018

A scalable screening of E. coli strains for recombinant protein expression

Luana Galvão Morão, Lívia Regina Manzine, Lívia Oliveira D. Clementino, Carsten Wrenger, Alessandro S. Nascimento

Citations: 25 • 2022

The RNA polymerase factory: a robotic in vitro assembly platform for high-throughput production of recombinant protein complexes

Sven Nottebaum, Lin Tan, Dominika Trzaska, Hannah C. Carney, Robert O. J. Weinzierl

Citations: 25 • 2007

An Engineered Survival-Selection Assay for Extracellular Protein Expression Uncovers Hypersecretory Phenotypes in <i>Escherichia coli</i>

Aravind Natarajan, Charles H. Haitjema, Robert Lee, Jason T. Boock, Matthew P. DeLisa

Citations: 24 • 2017

Automated Purification of His ₆ -Tagged Proteins Allows Exhaustive Screening of Libraries Generated by Random Mutagenesis

Thomas Lanio, Albert Jeltsch, Alfred Pingoud

Citations: 24 • 2000

Robotic Construction and Screening of Lanthipeptide Variant Libraries in <i>Escherichia coli</i>

Erpeng Guo, Lihao Fu, Xiaoting Fang, Wenhao Xie, Keyi Li, Zhiyu Zhang, Zhilai Hong, Tong Si

Citations: 18 • 2022