Enzyme

A Robot-Based Platform to Measure Multiple Enzyme Activities in Arabidopsis Using a Set of Cycling Assays: Comparison of Changes of Enzyme Activities and Transcript Levels during Diurnal Cycles and in Prolonged Darkness[W]

Yves Gibon, Oliver E. Blaesing, Jan Hannemann, Petronia Carillo, Melanie Höhne, Janneke Hendriks, Natalia Palacios‐Rojas, Joanna Cross, Joachim Selbig, Mark Stitt

Citations: 547 • 2004

A New Vector for High-Throughput, Ligation-Independent Cloning Encoding a Tobacco Etch Virus Protease Cleavage Site

Lucy Stols, Minyi Gu, Lynda Dieckman, Rosemarie Raffen, F. Collart, Mark I. Donnelly

Citations: 541 • 2002

A cell-free protein synthesis system for high-throughput proteomics

Tatsuya Sawasaki, Tomio Ogasawara, Ryo Morishita, Yaeta Endo

Citations: 523 • 2002

Identification of proteins directly from tissue: <i>in situ</i> tryptic digestions coupled with imaging mass spectrometry

M. Reid Groseclose, Malin Andersson, William M. Hardesty, Richard M. Caprioli

Citations: 372 • 2007

Dynamic Loading and Unloading of Proteins in Polymeric Stomatocytes: Formation of an Enzyme-Loaded Supramolecular Nanomotor

Loai K. E. A. Abdelmohsen, Marlies Nijemeisland, Gajanan M. Pawar, Geert-Jan Janssen, Roeland J. M. Nolte, Jan C. M. van Hest, Daniela A. Wilson

Citations: 304 • 2016

Temporal responses of transcripts, enzyme activities and metabolites after adding sucrose to carbon‐deprived Arabidopsis seedlings

Daniel Osuna, Björn Usadel, Rosa Morcuende, Yves Gibon, Oliver E. Bläsing, Melanie Höhne, Manuela Günter, Beate Kamlage, Richard N. Trethewey, Wolf‐Rüdiger Scheible, Mark Stitt

Citations: 300 • 2007

Vinyl Sulfones as Antiparasitic Agents and a Structural Basis for Drug Design

Iain D. Kerr, Ji Hae Lee, Christopher J. Farady, Rachael Marion, Mathias Rickert, Mohammed Sajid, Kailash C. Pandey, Conor R. Caffrey, Jennifer Legac, Elizabeth Hansell, James H. McKerrow, Charles S. Craik, Philip Rosenthal, Linda S. Brinen

Citations: 296 • 2009

Denitrification Response Patterns during the Transition to Anoxic Respiration and Posttranscriptional Effects of Suboptimal pH on Nitrogen Oxide Reductase in <i>Paracoccus denitrificans</i>

Linda Bergaust, Yuejian Mao, Lars R. Bakken, Åsa Frostegård

Citations: 271 • 2010

One-Step Purification of Recombinant Proteins Using a Nanomolar-Affinity Streptavidin-Binding Peptide, the SBP-Tag

Anthony D. Keefe, David Sloan Wilson, Burckhard Seelig, Jack W. Szostak

Citations: 264 • 2001

Protein expression systems for structural genomics and proteomics

Shigeyuki Yokoyama

Citations: 256 • 2003

Gliadin T Cell Epitope Selection by Tissue Transglutaminase in Celiac Disease

Burkhard Fleckenstein, Øyvind Molberg, Shuo‐Wang Qiao, Dietmar G. Schmid, Florian von der Mu ̈lbe, Katja Benedikte Prestø Elgstøen, Gu ̈nther Jung, Ludvig M. Sollid

Citations: 231 • 2002

Biomolecular robotics for chemomechanically driven guest delivery fuelled by intracellular ATP

Shuvendu Biswas, Kazushi Kinbara, Tatsuya Niwa, Hideki Taguchi, Noriyuki Ishii, Sumiyo Watanabe, Kanjiro Miyata, Kazunori Kataoka, Takuzo Aida

Citations: 216 • 2013

An improved method for the in vitro evolution of aptamers and applications in protein detection and purification

Michael Murphy

Citations: 202 • 2003

Periostin, a Member of a Novel Family of Vitamin K-dependent Proteins, Is Expressed by Mesenchymal Stromal Cells

Daniel L. Coutu, Jian Wu, Anne Monette, Georges‐Étienne Rivard, Mark Blostein, Jacques Galipeau

Citations: 187 • 2008

Antagonistic Enzymes in a Biocatalytic pH Feedback System Program Autonomous DNA Hydrogel Life Cycles

Laura Heinen, Thomas Heuser, Alexander A. Steinschulte, Andreas Walther

Citations: 180 • 2017

Machine Learning on a Robotic Platform for the Design of Polymer–Protein Hybrids

Matthew Tamasi, Roshan Patel, Carlos H. Borca, Shashank Kosuri, Heloise Mugnier, Rahul Upadhya, N. Sanjeeva Murthy, Michael E. Webb, Adam J. Gormley

Citations: 173 • 2022

Rapid optimization of enzyme mixtures for deconstruction of diverse pretreatment/biomass feedstock combinations

Goutami Banerjee, Suzana Car, John S. Scott‐Craig, Melissa S. Borrusch, Jonathan D. Walton

Citations: 170 • 2010

Self-driving laboratories to autonomously navigate the protein fitness landscape

Jacob Rapp, Bennett J. Bremer, Philip A. Romero

Citations: 169 • 2024

RESEARCH: Development of a mature fungal technology and production platform for industrial enzymes based on a <i>Myceliophthora thermophila</i> isolate, previously known as <i>Chrysosporium lucknowense</i> C1

Hans Visser, Vivi Joosten, Peter J. Punt, Alexander V. Gusakov, Phil T. Olson, Rob Joosten, Jeffrey Bartels, Jaap Visser, А. П. Синицын, Mark Emalfarb, Jan C. Verdoes, Jan Wery

Citations: 169 • 2011

Structures of the cIAP2 RING Domain Reveal Conformational Changes Associated with Ubiquitin-conjugating Enzyme (E2) Recruitment

Peter D. Mace, Katrin Linke, Rebecca Feltham, Frances‐Rose Schumacher, Clyde A. Smith, David L. Vaux, John Silke, Catherine L. Day

Citations: 168 • 2008