Yan Nie

Unit of Virus Host Cell Interactions, European Molecular Biology Laboratory

Papers

Total Citations

248

H-Index

About

Yan Nie is a leading figure in recombinant protein production and molecular tool development, with a career dedicated to advancing the study of multiprotein complexes. Her primary research areas encompass baculovirus expression systems, synthetic biology cloning strategies, and high-throughput protein complex assembly. Nie’s seminal 2010 work, “New baculovirus expression tools for recombinant protein complex production,” has garnered over 220 citations, establishing a foundational platform for producing challenging multi-subunit proteins in insect cells. She further revolutionized the field with her 2013 protocol on “Tandem Recombineering by SLIC Cloning and Cre-LoxP Fusion,” which provides a robust, stepwise method for generating multigene expression constructs. By ingeniously combining sequence and ligation independent cloning (SLIC) with Cre-LoxP recombination, Nie enabled researchers to efficiently assemble complex DNA cassettes for multiprotein studies. Her protocol, detailed with PCR-based strategies, has become a critical resource for structural biologists and biochemists. Through these contributions, Yan Nie has significantly lowered the technical barriers to studying macromolecular machines, empowering laboratories worldwide to probe the architecture and function of essential protein complexes.