Sophia Hober
Papers
2
Total Citations
41
H-Index
2
About
Sophia Hober is a leading figure in protein engineering and proteomics, renowned for developing innovative tools that bridge the gap between antibody-based capture and mass spectrometry. Her key research areas include affinity tag design, high-throughput protein purification, and quantitative proteomics. Hober’s major contribution is the creation of the immuno-SILAC method, a groundbreaking approach that combines polyclonal antibodies with stable isotope-labeled recombinant proteins for absolute protein quantification in complex samples (31 citations). This work has significantly advanced the precision of targeted proteomics. She also pioneered a high-throughput purification strategy using the polycationic Z(basic) tag under denaturing conditions, enabling efficient recovery of both soluble and insoluble proteins via cation exchange chromatography (10 citations). This innovation has streamlined protein production for structural and functional studies. Beyond these achievements, Hober’s work on affinity ligands and recombinant antibody fragments has had lasting impact on bioprocessing and diagnostic development. Her contributions are widely cited and continue to inspire new methods in quantitative biology and protein engineering.
Research Focus
Key Achievements
Top Papers
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