Mutagenesis

Ultrahigh-throughput screening in drop-based microfluidics for directed evolution

Jeremy J. Agresti, Eugene Antipov, Adam R. Abate, Keunho Ahn, Amy C. Rowat, Jean‐Christophe Baret, Manuel Márquez, Alexander M. Klibanov, Andrew D. Griffiths, David A. Weitz

引用数: 1147 • 2010

High resolution functional analysis of antibody-antigen interactions

Lei Jin, B M Fendly, James A. Wells

引用数: 213 • 1992

Industrial strain improvement: Mutagenesis and random screening procedures

Robert T. Rowlands

引用数: 164 • 1984

Directed Evolution of an Esterase from Pseudomonas fluorescens. Random Mutagenesis by Error-Prone PCR or a Mutator Strain and Identification of Mutants Showing Enhanced Enantioselectivity by a Resorufin-Based Fluorescence Assay

Erik Henke, Uwe T. Bornscheuer

引用数: 103 • 1999

Protein engineering via Bayesian optimization-guided evolutionary algorithm and robotic experiments

Ruyun Hu, Lihao Fu, Yongcan Chen, Junyu Chen, Yu Qiao, Tong Si

引用数: 60 • 2022

Gel microdroplet–based high-throughput screening for directed evolution of xylanase-producing Pichia pastoris

Chunxuan Ma, Zheng Lin Tan, Ying Lin, Shuangyan Han, Xin‐Hui Xing, Chong Zhang

引用数: 53 • 2019

Heterologous expression of Melanocarpus albomyces cellobiohydrolase Cel7B, and random mutagenesis to improve its thermostability

Sanni Voutilainen, Harry Boer, Markus B. Linder, Terhi Puranen, Juha Rouvinen, Jari Vehmaanperä, Anu Koivula

引用数: 45 • 2007

Screening and genetic characterization of thermo-tolerant Synechocystis sp. PCC6803 strains created by adaptive evolution

Ulrich M. Tillich, Nick Wolter, Philipp Franke, Ulf Dühring, Marcus Frohme

引用数: 35 • 2014

Comprehensive Discovery of Cell-Cycle-Essential Pathways in <i>Chlamydomonas reinhardtii</i>

Michal Breker, Kristi Lieberman, Frederick R. Cross

引用数: 35 • 2018

The Bridge Helix of RNA Polymerase Acts as a Central Nanomechanical Switchboard for Coordinating Catalysis and Substrate Movement

Robert O. J. Weinzierl

引用数: 27 • 2011

The RNA polymerase factory: a robotic in vitro assembly platform for high-throughput production of recombinant protein complexes

Sven Nottebaum, Lin Tan, Dominika Trzaska, Hannah C. Carney, Robert O. J. Weinzierl

引用数: 25 • 2007

The robotic mouse: Unravelling the function of AF4 in the cerebellum

Emmanuelle Bitoun, Kay E. Davies

引用数: 24 • 2005

Automated Purification of His ₆ -Tagged Proteins Allows Exhaustive Screening of Libraries Generated by Random Mutagenesis

Thomas Lanio, Albert Jeltsch, Alfred Pingoud

引用数: 24 • 2000

Fast and efficient template-mediated synthesis of genetic variants

Liyuan Liu, Yiming Huang, Harris H. Wang

引用数: 19 • 2023

Efficient screening for new amino acid dehydrogenase activity: Directed evolution of Bacillus sphaericus phenylalanine dehydrogenase towards activity with an unsaturated non-natural amino acid

Sihong Chen, Paul C. Engel

引用数: 19 • 2009

Cost-Effective High-Throughput Fully Automated Construction of a Multiplex Library of Mutagenized Open Reading Frames for an Insecticidal Peptide Using a Plasmid-Based Functional Proteomic Robotic Workcell with Improved Vacuum System

Stephen R. Hughes, Patrick F. Dowd, Ronald E. Hector, Steven B. Riedmuller, Scott Bartolett, Jeffrey A. Mertens, Nasib Qureshi, Siqing Liu, Kenneth M. Bischoff, Xin-Liang Li, John S. Jackson, David Sterner, Tadas Panavas, Joseph O. Rich, Philip J. Farrelly, Tauseef R. Butt, Michael A. Cotta

引用数: 16 • 2007

Rational mutagenesis to support structure-based drug design: MAPKAP kinase 2 as a case study

M.A. Argiriadi, Silvino Sousa, David Banach, D.J. Marcotte, Tao Xiang, Medha J. Tomlinson, Megan D. Demers, Christopher M. Harris, Silvia Kwak, Jennifer Hardman, Margaret Pietras, Lisa Quinn, J. Dimauro, Baofu Ni, John A. Mankovich, David W. Borhani, Robert V. Talanian, Ramkrishna Sadhukhan

引用数: 11 • 2009

Plasmid-Based One-Pot Saturation Mutagenesis and Robot-Based Automated Screening for Protein Engineering

Fumihiro Kawai, Akihiko Nakamura, Akasit Visootsat, Ryota Iino

引用数: 8 • 2018

Droplet-Based Microfluidic High-Throughput Screening of Enzyme Mutant Libraries Secreted by Yarrowia lipolytica

Thomas Beneyton, Tristan Rossignol

引用数: 8 • 2021

Automating Gene Library Synthesis by Structure-Based Combinatorial Protein Engineering

Melissa Dokarry, Caroline Laurendon, Paul E. O’Maille

引用数: 8 • 2012