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Anahita Safavi-Naini is a prominent researcher in synthetic biology and microbial genetics, best known for pioneering high-throughput genetic interaction mapping in bacteria. Her landmark 2008 paper, "eSGA: E. coli synthetic genetic array analysis," has garnered over 250 citations, establishing a foundational method for systematically probing gene function and epistasis in *Escherichia coli*. This work introduced an automated, array-based approach to construct double mutants, enabling large-scale identification of synthetic lethal and buffering interactions—a critical tool for understanding bacterial cellular networks and antibiotic target discovery. Safavi-Naini’s contributions have significantly advanced our ability to dissect complex genetic architectures, with implications for both fundamental biology and applied synthetic biology. Her research bridges computational and experimental design, offering a scalable framework for functional genomics. Beyond her citation impact, she has been recognized for developing open-source resources that democratize synthetic genetic array analysis, empowering labs worldwide to explore bacterial gene function. Her work continues to inspire new strategies for engineering microbial systems and combating drug resistance.