Guidance of cellular nematics into shape-programmable living surfaces

Abstract

Abstract Engineering living materials capable of autonomously morphing into predetermined shapes holds great potential for applications ranging from synthetic tissue morphogenesis to soft robotics. In this regard, harnessing the inherent ability of cellular tissues to self-organize and generate robust force fields offers a promising strategy for creating self-shaping living surfaces. However, achieving precise control over tissue mechanics to direct specific morphogenetic outcomes remains a challenge. Here, we introduce a strategy to program tissue shape transformations through the nematic organization of cellular forces. By precisely controlling nematic order and topological defects, we generate millimeter-scale tissues programmed with specific multicellular tension fields. Using a theoretical framework that integrates contractile nematics with thin sheet mechanics, we explore the role of nematically guided tensions in shape morphogenesis. Experimentally, upon tissue detachment, nematically guided tension fields drive out-of-plane deformations, generating reproducible 3D shapes. By integrating tissue contractility and nematic patterning, our approach offers a robust framework for the rational design of shape-programmable living surfaces.