An Integrated Proteomic Workflow for Two-Dimensional Differential Gel Electrophoresis and Robotic Spot Picking

Abstract

New technologies have advanced the field of proteomics, and a number of companies have developed innovative platforms to drive this research. However, significant challenges are often encountered when trying to integrate complementary technologies from multiple manufacturers. We have developed a software and hardware solution to integrate the Ettan two-dimensional difference gel electrophoresis (2-D DIGE) system (GE Healthcare) with the Investigator ProPic spot picking robot (Genomic Solutions). We have analyzed protein sample preparations from bacterial and mammalian sources to demonstrate a new workflow with increased throughput for gel-based proteomics. Keywords: 2-D DIGE • ProPic Workstation • Yersinia pestis • high-throughput • proteome • gel-based proteomics • saturation labeling • minimal labeling • CyDye • integration