Automated high-throughput purification of genomic DNA from whole blood using Promega's MagneSil^TMparamagnetic particles with either the Max Yield or MagneSil^TMONE normalized purification methods

摘要

Two different methods of automated high throughput purification of genomic DNA from human whole blood in 96 well plates are described. One method uses MagneSil^TM paramagnetic particles to purify a maximal amount of the DNA present in the sample. Another method, the Magnesil^TM ONE system, allows for the purification of a predetermined amount of DNA from human whole blood. Protocols for the purification of 100 ng or, alternatively 1 ug, of human genomic DNA from whole blood using MagneSil^TM paramagnetic particles and a Beckman BioMek^TM FX robot are described. With the maximal yield purification system, typical DNA yields fall in the range of 4-9 ug of DNA from 200ul of human whole blood, depending upon the white cell content of donor sample. For situations where DNA achiving is desired, or when the number of downstream sample applications is not clearly defined (e.g. multiple SNP analyses) the maximal yield method is usually preferred. However, in situations with a defined downstream application (e.g. criminal databasing or use of a defined set of amplifications) where purifying DNA in a narrow concentrate range streamlines the high throughput purification and analysis process, the automated MagneSil^TM ONE purification system is the method of choice. DNA from either method is suitable for applications such as PCR, STR, READIT^TM SNP analysis, and multiplexed PCR systems such as Promega's Y-chromosome deletion detection system.