Validation of a High-Throughput Automated Liquid Handling DNA Extraction System to Maximize Efficiency in Forensic Casework

摘要

Background: Decreasing turnaround times and reducing the backlog for criminal cases containing biological evidence remains a critical priority for public safety. In the forensic crime laboratory, one strategy to increase efficiency and capacity is to incorporate robotic instrumentation. In addition to increasing sample throughput, automation also decreases sources of variability and error compared with manual methods, while enabling analysts to focus on higher value activities. Here, we present the validation of a high throughput solution for DNA extraction and purification from forensic evidence type samples using the Hamilton Microlab® VANTAGE Liquid Handling System.® Methods: Sensitivity, reproducibility, contamination, and nondifferential and differential mock evidence studies were all performed according to the SWGDAM Validation Guidelines, using both VANTAGE and QIAsymphony SP liquid handling platforms. Data obtained using each automated system were compared. Results: Samples (i.e. blood, saliva, semen) that ere purified on the VANTAGE generated accurate, sensitive, and reproducible quantification and profile results that were free from contamination regardless of the substrate (i.e. cotton, wood, metal, ceramic) from which the DNA was extracted. The extracted DNA yield produced by both the VANTAGE and the QIAsymphony SP were comparable. However, the VANTAGE processed up to four microplates (384 samples) in approximately 1 hour and 33 minutes, executing the workflow up to 10-fold faster than the QIAsymphony SP. Conclusions: In current laboratory environments where the influx of DNA sample submissions often outpaces laboratory capability, the VANTAGE high-throughput solution offers the forensic analyst with a means to maximize sample processing efficiency without compromising process quality.