B D Pfrogner
Papers
1
Total Citations
130
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1
About
B. D. Pfrogner is a molecular biologist whose foundational work in genomic library construction has enabled large-scale human genome analysis. Their primary research area involves developing and optimizing cloning systems for high-molecular-weight DNA, with a particular focus on the bacteriophage P1 system. Pfrogner’s most significant contribution is the construction and screening of an arrayed human genomic library with threefold coverage of the haploid genome, detailed in a 1994 paper that has garnered over 130 citations. This work provided a critical resource for mapping and sequencing efforts by offering stable, large-insert clones that could be systematically screened. By size-fractionating Sau3AI partial digests, Pfrogner ensured high-quality, representative coverage of the genome, facilitating subsequent gene isolation and physical mapping projects. This achievement remains a cornerstone in genomic tool development, demonstrating Pfrogner’s lasting impact on the field. Their meticulous approach to library preparation set standards for reproducibility and accessibility, making them a key figure in the early era of human genomics.
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