An Automated Analytical High-Performance Liquid Chromatographic Procedure for Iopamidol Solutions Using a Benchmate Workstation

Abstract

Abstract A flexible compact robot system for sample work-up and preparation of calibration standards for HPLC analyses is presented. The equipment consisting of a BenchMate workstation and a conventional HPLC system was used to analyse aqueous solutions containing iopamidol, a contrast medium. The analytical procedure was characterized with respect to sensitivity, selectivity and linearity of the detector response. The use of the workstation yielded excellent data with respect to accuracy and intra-assay precision. The application could be used after few modifications to determine other compounds in solution or microcrystalline suspensions. The automatization of this kind of analysis saved man-hours (ca. 25%) and could reduce the costs of glassware and of solvents. An additional advantage is the reduced individual exposition to solvent vapour and other chemicals.