Automation Protocol for Plasmid DNA Extraction from E. coli v1

Abstract

Here, we describe a protocol for automated extraction of plasmid DNA from 24 E. coli cultures. The protocol uses an automated liquid handler and a positive pressure filter press. It is based on the QIAprep Spin Miniprep Kit (Qiagen, 27106). This protocol requires an automated liquid handler (Hamilton Robotics, STAR) with both 8-channel and 96-channel pipetting heads and positive pressure filter press (Hamilton, MPE2). It also requires a centrifuge with a 96-well plate rotor capable of at least 3878 g.