An Automated 1536-Well Microplate Format Cytochrome P450 Inhibition Assay Using a Tecan Freedom EVO Workstation with Integrated Innovadyne Nanodrop II Dispenser

Abstract

A Tecan EVO Workstation and Innovadyne Nanodrop II liquid dispenser have been integrated to provide an automated miniaturized cytochrome P450 inhibition assay, using 1536-well plate technology. The Tecan EVO was used to perform larger volume bulk reagent and compound dilution operations along with plate manipulations using the Tecan Robotic Manipulator. All reagent additions to the 1536-well microplates were performed exclusively by the Nanodrop dispenser, which is capable of accurate and precise pipetting at volumes as low as 100 nL. Miniaturization from 96- to 1536-well plate formats has enabled a fourfold increase in P450 inhibition assay capacity, while reducing reagent costs by approximately 20-fold.