StretchfMRI: a novel technique to quantify the contribution of the reticular formation to long-latency responses via fMRI

Abstract

Increased reticulospinal (RS) function has been observed to cause both positive and negative outcomes in the recovery of motor function after corticospinal lesions such as stroke. Current knowledge of RS function is limited by the lack of accurate, noninvasive methods for measuring RS function. Recent studies suggest that the RS tract may be involved in processing and generating Long Latency Responses (LLRs). As such, LLRs, elicited by applying precisely controlled perturbations, can thus act as a reliable stimulus to measure brainstem function using fMRI with high signal-to-noise ratio.In this paper, we present StretchfMRI, a novel technique that enables simultaneous recording of neural and muscular activity during motor responses conditioned by robotic perturbations, which allows direct investigation of the neural correlates of LLRs.Via preliminary validation experiments, we demonstrate that our technique can reliably elicit and identify LLRs in two wrist muscles-Flexor Carpi Radialis and Extensor Carpi Ulnaris. Moreover, via a single-subject pilot experiment, we show that the occurrence of an LLR in a flexor and extensor muscles modulates neural activity in distinct regions of the brainstem. The observed somatotopic organization is in agreement with the double reciprocal model of RS function observed in animal models, in which the right medullary and left pontine reticular formation are responsible for control of the motor activity in flexors and extensors, respectively.