Supported liquid membrane extraction of urinarytrans, trans-muconic acid, a biomarker for benzene exposure

Abstract

In this study, sample preparation of urinary trans, trans-muconic acid (tt-MA), a minor metabolite and biomarker of benzene, by means of Supported Liquid Membrane (SLM) extraction has been investigated. The sample extract was automatically transferred on-line to an ion exchange chromatographic column and the analytes were detected using a photodiode array detector. Automation of the system was accomplished using a robotic sample preparation processor integrated into the chromatographic system. Detection limits calculated from the baseline noise was in the sub-ppb range, and quantification of tt-MA is safe down to 10 ppb.