An Automated p-Nitrophenylphosphate Serum Alkaline Phosphatase Procedure for the "Robot Chemist"

Abstract

Abstract A new, precise, and accurate procedure is described for the automated determination of serum alkaline phosphatase on the Robot Chemist. The substrate, p-nitrophenyl-phosphate in 2-amino-2-methyl-1-propanol, offers the prime advantage of providing directly its own chromogen, p-nitrophenol, following enzyme action. Other advantages are the small sample volumes required and linear reaction rates. Correlations are presented between the manual procedure and those used with both the Technicon Auto-Analyzer and the Robot Chemist.