New version of<i>CrysAlisPro</i>optimized for automatic macromolecular data collection and processing

Abstract

The handling of cryo-cooled protein crystals has been automated by robotics systems, where samples are kept in liquid-nitrogen storage, to be loaded on the goniometer for the experiment and retrieved afterwards. Crystals are automatically centered in the X-ray beam by means of an automated goniometer head and software that applies algorithms that determine the crystal position in three-dimensional space from images taken with high resolution digital microscopes. For the steps that follow, we report on the development of XPRESSO, a new crystal screening and data collection system for macromolecular samples.