The Automated Determination of Serum Uric Acid

Abstract

Abstract An automated procedure is presented for the enzymatic determination of serum uric acid on both the AutoAnalyzer and the Robot Chemist. The procedure measures as the neocuproine complex, the difference in the amount of Cu+ formed by reaction of a Cu++-alkanolamine buffered solution with serum uric acid under precisely controlled conditions before and after uricase treatment of the serum. The difference is proportional to the true serum uric acid content. The elements contributing to the enzymatic reaction, the colorimetric reaction, and the elimination of interferences were investigated. Comparison of serum uric acid values obtained by this method with those obtained by ultraviolet spectrophotometry show very good agreement.