Short Column Chromatography for a Robotic Dissolution Assay of Adinazolam Mesylate Tablets

摘要

The use of robotics can potentially increase the productivity of pharmaceutical quality control laboratories, especially in areas such as dissolution testing. However, if the speed of the assay is limited by factors other than the speed of the robot, such as chromatography, the productivity of the assay is less than optimal. A robotic dissolution assay for adinazolam mesylate tablets is currently under development. One of the obstacles to implementation is that the productivity of the assay is limited by a chromatographic analysis time of six minutes per sample. This report descibes the application of short column chromatography to reduce the analysis for dissolved drug to under two minutes. Three short columns were evaluated for use in dissolution rate assay of adinazolam mesylate tablets. In this case, the application of short column technology allows for a potential increase in productivity of about 75 percent. Similar productivity gains may be achieved for other robotic assays that are limited by the speed of the chromatography.