A fast and convenient method for robotic preparation of [11C]flumazenil avoiding HPLC purification

摘要

A new approach for robotic preparation of [11C]flumazenil with recovery of the final product by SPE technique has been developed. A hydrogen-bond-assisted methylation in a suspension of potassium fluoride absorbed on alumina was followed by purification using a standard Sep-Pak Neutral Alum N Light cartridge. As a result, the contamination of the final product with its precursor Ro 15-5528 was reduced to similar levels as obtained by traditional semi-preparative HPLC. The synthesis of [11C]flumazenil was operated by the commercially available robot Anatech RB-86. The formulated solution of [11C]flumazenil was obtained within 18 min starting from trapping of [11C]methyl iodide. The simplicity of this method and the use of disposable materials for purification instead of HPLC may be advantageous for routine clinical use. Copyright © 2000 John Wiley & Sons, Ltd.