Optimization of a Liquid Chromatographic Method Based on Information Theory: Application of the Method to Dissolution Testing

摘要

Abstract The simultaneous optimization of mobile phase composition, column length, detection wavelength, and flow rate was applied to the development of an assay method for dissolution testing of multi-ingredient formulations. This optimization procedure is called Total Chromatographic Optimization and is based on the information theory of chromatography. Optimal chromatographic conditions resulted in very rapid and precise measurements. The dissolution test procedures, including sampling, dilution, filtering, and assay, were performed automatically by a laboratory robotic system. The dissolution medium was sampled every 15 min and analyzed immediately for drug concentration.