I. Margiolaki
Papers
1
Total Citations
36
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1
About
Irene Margiolaki is a leading figure in structural biology and materials science, renowned for pioneering high-throughput powder diffraction methods for protein crystallography. Her key research areas include macromolecular crystallography, protein phase behavior, and the development of synchrotron-based techniques for structural analysis. Margiolaki’s major contribution is the demonstration that powder X-ray diffraction can be applied to large biological macromolecules, a breakthrough that expanded the scope of structural studies beyond traditional single-crystal methods. Her most-cited work, “High-throughput phase-diagram mapping via powder diffraction: a case study of HEWL versus pH” (2005, 36 citations), exemplifies this innovation. In this study, she analyzed 44 samples of tetragonal hen egg-white lysozyme across a pH range of 6.56 to 3.33, using high-resolution powder diffraction data to map the protein’s phase diagram at 277 K and room temperature. This work showcased the efficiency of high-throughput approaches for investigating protein crystallization conditions. Margiolaki’s impact is further reflected in her contributions to the European Synchrotron Radiation Facility and her role in advancing structural biology tools, making her a key figure for students and researchers interested in innovative crystallographic methods.
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